Fig. 4

Overexpression of N-glycosylation defective CD147 mutant N152Q blocked MMPs production in BMECs. a Representative Western blots of CD147, caveolin-1, occludin, and ZO-1. b, c Densitometric quantification results of CD147 and caveolin-1 in control and empty vector- or N152Q mutant-transfected BMECs. d, e MMP-2/9 activity in the conditioned media. f, g Densitometric quantification results of occludin and ZO-1. Data expressed as mean ± SEM and analyzed by one-way ANOVA. Asterisk (*) denotes comparison with the control group and pound (#) with the empty vector group. One asterisk (*) represents P < 0.05, two (**) P < 0.01, and three (***) P < 0.001. EV empty vector, BMEC brain microvascular endothelial cell, MMP matrix metalloproteinase, ZO-1 zonula occludens-1