Fig. 6

Caveolin-1 binds to CD147 and upregulates its glycosylation. a CD147 and caveolin-1 co-localize in astrocytes and endothelium. b Caveolin-1 silencing by siRNA downregulates HG-CD147 in astrocytes and endothelial cells treated with AGEs and rt-PA. c, d Decrease of HG-CD147 reduces MMPs activities. Data expressed as mean ± SD and analyzed by one-way ANOVA test. One asterisk (*) represents P < 0.05, two (**) P < 0.01, and three (***) P < 0.001. e HEK293 cells were transfected with HA-caveolin-1 and FLAG-CD147 constructs. The whole cell lysates were subjected to immunoblot analysis (input). In addition, the whole cell lysates were first immunoprecipitated with anti-HA antibody and then detected with anti-CD147 antibody (IP:HA). ASTRO astrocyte, ENDO endothelial cell; HG highly glycosylated, LG lowly glycosylated, MMP matrix metalloproteinase, CO-IP co-immunoprecipitation