Fig. 2

PK system activation in the sciatic nerve. a Representative images of PK2 immunofluorescence signal in the sciatic nerve sections of CTR, BTZ 14 days (corresponding to c.d. 2.4 mg/kg), BTZ 28 days (corresponding to c.d. 4.8 mg/kg), and PC1 (BTZ + PC1) mice. Cell nuclei were counterstained with DAPI (blue fluorescence). Quantitative analysis of PK2 signal (b) was computed as integrated optical density for arbitrary areas (6 sections per animal, 5 animals per group). One-way ANOVA was used for statistical evaluation, followed by Bonferroni’s test for multiple comparisons. ***p < 0.001 vs CTR; °°°p < 0.001 vs BTZ day 28. c, d mRNA levels of PK-R1 and PK-R2 respectively, measured by real-time qPCR, 14 days after the first BTZ administration (c.d. 2,4 mg/kg) and at the end of BTZ protocol (c.d. 4.8 mg/kg, day 28). Data represent mean ± SD of 6 mice/group. Statistical analysis was performed by mean of one-way ANOVA