Fig. 6

Enlarged pericytes highly localized to areas of fibrinogen extravasation and pericyte infection IF imaging for PDGFRB (red), SIVp28 (green), and DAPI (blue) a of 10 vessels with and 10 without enlarged pericyte coverage for each SIVE animal (n = 6) showed that 100% of the enlarged pericytes (n = 60) had localized SIVp28+ staining, while only 8% of the non-enlarged pericytes (n = 60) showed positive staining (b). Five-color IF staining for GFAP (gold), PDGFRB (red), GLUT1 (aqua), SIVp28 (green), and DAPI (dark blue) show the major cellular components of the glio-vascular interface and how they localize with SIVp28 viral protein marker in the non-hypertrophied pericyte of an uninfected animal (11A014) and the hypertrophied pericyte of an SIVE animal (11A554) (c, d). This demonstrates the presence of SIVp28 primarily in the pericytes and endothelial cells (d). Presence of SIVp28+ staining within pericytes was confirmed via a confocal microscopy Z-stack of a 20-μm-thick section using 0.25 μm increments analyzed with a ZenBlack co-localization test in which points in quadrant 3 and highlighted in yellow are considered to be co-localized between PDGFRB and SIVp28 (e). A paired t test was used to determine significance between groups in b