Fig. 2

Effects of 1810034E14Rik on the biological function of microglial cells. a Primary microglia were transfected with 1810034E14Rik or siRNA-1810034E14Rik for 24 h and then challenged with 4 h OGD/24 h reperfusion to induce an ischemic state. Immunofluorescence staining of Iba1. b RT-qPCR validation of the expression of lncRNA-1810034E14Rik. c CCK-8 analysis of the viability of microglial cells. d, e RT-qPCR validation of the expression of CD11b d and CD16 e. f Cell migration of microglia was measured by scratch assay, and wound closure at 18 h was used to compare cell motility. g 5 μm red fluorescent microspheres (10⁶/ml) were added to culture to observe the phagocytosis of the microglia. h Quantification of Fig. 2a. i Quantification of Fig. 2f. j Quantification of Fig. 2g. The data represents mean ± SEM. n = 15, *P < 0.05, **P < 0.01, and ***P < 0.001 versus the control group; ##P < 0.01, ###P < 0.001 versus the OGD + Lv-control group; ^P < 0.05, ^^^P < 0.001 versus the OGD + siControl group