Fig. 2
Myosin1f is required for neutrophil migration after acute ischemic stroke. A Myosin1f gene expression increased in blood neutrophils at 3 days after stroke compared to the sham control group(s), **** p < 0.0001, n = 3 mice/group; each has 3 replicates. B In vitro migration assay: the number of myosin1f +/+ neutrophils migrated from the upper chamber to the lower chamber was significantly higher compared to myosin1f−/− neutrophils. *p = 0.0463, n = 3–4 mice/group; each has 3 replicates. C Confocal immunofluorescent staining for MPO, which was counterstained with DAPI. (a) A representative brain section stained with cresol/violet is presented to show the three indicated regions where cell numbers were quantified. (b) Representative staining of MPO+ (green) and DAPI (blue) at 72 h after stroke. (c) The bar graph represents the statistic results of the MPO+ positive cells. Scale bar: 50 μm. ** p = 0.0024, n = 5–6 mice/group. D FACS quantification of infiltrating neutrophils in the ischemic brain. (a) The representative gating strategies of CD45+, MiDMs (microglia-derived macrophages, CD45LowCD11b+), MoDM (monocytes-derived macrophages, CD45HiCD11b+), and neutrophils (CD11b+Ly6G+). (b) The cell numbers of total CD45+ leukocytes, neutrophils, MiDMs, and MoDMs. (*, **, ***, ****, p < 0.05, 0.01, 0.001, 0.0001, respectively. N = 3–5 mice/group.)