Fig. 4

Absence of M2 switching in DOCA-salt rats. a Microglia and macrophages were visualized by Iba-1 (red) and CD206 (M2 marker, green) in the control (left column), DOCA2W (middle column), and DOCA3W (right column). The white dotted square in the upper row is magnified in the lower row. CD206-positive microglia were not observed in DOCA-salt rats (open arrowheads). In the white square, only Iba-1 staining was noted. CD206-positive perivascular macrophages were present in all periods (arrows). b, c No significant increase in the expression level of arginase-1 protein was observed after DOCA and salt treatment. Scale bars 50 μm