Fig. 4
Effects of MIF inhibitor 4-IPP on the expression of PGE2 synthesis-related protein. a Western blot analysis of COX1, COX2, mPGES-1, mPGES-2, and cPGES following astrocytes treatment with 1 μg/ml recombinant MIF for 24 h in the presence of 100 μM 4-IPP. b–d Quantification data as shown in (a). e, f Cell supernatants (e) and lysates (f) were tested by ELISA for the production of PGE2, following astrocytes treatment with 0–2.5 μg/ml recombinant MIF for 24 h in the presence of 100 μM 4-IPP. Experiments were performed in triplicates. Error bars represent the standard deviation (*P < 0.05; #P < 0.05)