Fig. 4

ICA depressed glial cells activation through activating Nrf2 signing. WT and Nrf2 KO mice were treated with ICA (60 mg/kg/d) with a single intranigral injection of 6-OHDA (4 μg) in the SN on the left side of the brain beginning 3 days after ICA treatment. Later, brain sections from WT and Nrf2 KO mice were immunostained with anti-Iba-1 (a) and GFAP (c) antibodies, respectively. The protein expressions of Iba-1 (b) and GFAP (d) in WT and Nrf2 KO mice were measured by western blotting. Results were mean ± SEM from 5 to 6 mice. *p < 0.05 compared with the control group, ^#p < 0.05 compared with 6-OHDA-treated group. Scale bar = 100 μm