Table 4 Ultrastructural analysis of microglial processes in healthy (APP-H), dystrophic (APP-D), and plaque and dystrophy (APP-P) containing regions of APPSwe-PS1Δe9 mice
From: Ultrastructural evidence of microglial heterogeneity in Alzheimer’s disease amyloid pathology
APP-H | APP-D | APP-P | |
|---|---|---|---|
Mean ± SEM | Mean ± SEM | Mean ± SEM | |
Area (μm2) | 0.361 ± 0.026 | 0.451 ± 0.033 | 0.485 ± 0.037 |
Perimeter (μm) | 3.333 ± 0.197 | 3.575 ± 0.2 | 3.317 ± 0.209 |
Circularity (a.u.) | 0.486 ± 0.016 | 0.501 ± 0.017 | 0.488 ± 0.018 |
Roundness (a.u.) | 0.485 ± 0.014 | 0.527 ± 0.014 | 0.492 ± 0.016 |
Solidity (a.u.) | 0.768 ± 0.012 | 0.789 ± 0.012 | 0.775 ± 0.125 |
Lipidic inclusion (n) | 0.295 ± 0.050 | 0.225 ± 0.046 | 0.487 ± 0.07## |
Fibrillar materials inclusion (n) | 0 | 0 | 0.047 ± 0.027~# |
Phagocytic process (%) | 0.2 ± 0.028 | 0.255 ± 0.031 | 0.24 ± 0.035 |
Total inclusion (n) | 0.295 ± 0.046 | 0.4 ± 0.056 | 0.38 ± 0.687 |
Non-empty inclusion (n) | 0.115 ± 0.029 | 0.19 ± 0.038 | 0.193 ± 0.041 |
Empty inclusion (n) | 0.18 ± 0.035 | 0.21 ± 0.04 | 0.147 ± 0.038 |
ER dilation (n) | 0.025 ± 0.011 | 0.05 ± 0.154 | 0.033 ± 0.0175 |
Extracellular debris (n) | 0.24 ± 0.04 | 0.255 ± 0.039 | 0.24 ± 0.047 |
Total lysosome (n) | 0.005 ± 0.005 | 0.002 ± 0.01 | 0.0933 ± 0.04~ |
Primary lysosome (n) | 0 | 0.02 ± 0.016 | 0 |
Secondary lysosome (n) | 0.005 ± 0.005 | 0.01 ± 0.007 | 0.06 ± 0.027~ |
Tertiary lysosome (n) | 0 | 0.01 ± 0.007 | 0.033 ± 0.024 |
Encircled Aβ (n) | 0 | 0 | 0.047 ± 0.027~# |
Encircled synaptic element (n) | 0.345 ± 0.067 | 0.215 ± 0.049 | 0.14 ± 0.038~ |
Encircled dendritic spine (n) | 0.185 ± 0.038 | 0.115 ± 0.028 | 0.08 ± 0.026 |
Encircled axonal terminal (n) | 0.155 ± 0.028 | 0.09 ± 0.024 | 0.053 ± 0.018 |
Encircled degraded structure (n) | 0.025 ± 0.013 | 0.04 ± 0.017 | 0.006 ± 0.006 |