Fig. 9

The knockdown of FoxO1 enhanced the inhibitory effect of baicalin on LPS-induced TLR4 expression and inflammation. a–d The knockdown of FoxO1 expression by FoxO1 siRNA. a The cells were stained with FoxO1 (green) antibody and observed using fluorescence microscopy (scale bar 100 μm). b Western blotting analysis of the expression of FoxO1 after the suppression of FoxO1 expression. c Western blotting analysis of the expression of p-PI3K, p-AKT, and TLR4 in LPS-stimulated primary microglia after transfection with FoxO1 siRNA and treated or not treated with baicalin. The levels of p-PI3K, p-AKT, and TLR4 were quantified. Data were normalized to the medium group. d The levels of IL-1β, IL-6, and TNF-α in the cell culture supernatants of LPS-stimulated primary microglia were determined by ELISA after transfection with FoxO1 siRNA. All of the results are presented as means ± SEM. ^#p < 0.05, ^##p < 0.01 vs. medium; *p < 0.05, **p < 0.01 vs. LPS; ^&p < 0.05, ^&&p < 0.01 vs. LPS+BA60; ^$$$p < 0.005 vs. LPS