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Fig. 9 | Journal of Neuroinflammation

Fig. 9

From: Baicalin ameliorates neuroinflammation-induced depressive-like behavior through inhibition of toll-like receptor 4 expression via the PI3K/AKT/FoxO1 pathway

Fig. 9

The knockdown of FoxO1 enhanced the inhibitory effect of baicalin on LPS-induced TLR4 expression and inflammation. ad The knockdown of FoxO1 expression by FoxO1 siRNA. a The cells were stained with FoxO1 (green) antibody and observed using fluorescence microscopy (scale bar 100 μm). b Western blotting analysis of the expression of FoxO1 after the suppression of FoxO1 expression. c Western blotting analysis of the expression of p-PI3K, p-AKT, and TLR4 in LPS-stimulated primary microglia after transfection with FoxO1 siRNA and treated or not treated with baicalin. The levels of p-PI3K, p-AKT, and TLR4 were quantified. Data were normalized to the medium group. d The levels of IL-1β, IL-6, and TNF-α in the cell culture supernatants of LPS-stimulated primary microglia were determined by ELISA after transfection with FoxO1 siRNA. All of the results are presented as means ± SEM. #p < 0.05, ##p < 0.01 vs. medium; *p < 0.05, **p < 0.01 vs. LPS; &p < 0.05, &&p < 0.01 vs. LPS+BA60; $$$p < 0.005 vs. LPS

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