Fig. 5

Motor activity recovery after glutamate administration in NOX-2 KO mice. Performance of wild-type (WT + SS) animals and WT or NOX-2 KO mice injected with glutamate (WT + GLUT; NOX-2 KO + GLUT) was evaluated in three tests of motor behavior at 1, 3, 6, 12, and 24 h after glutamate intracerebral injection. These tests were later integrated to obtain a motor score that reflects functional deterioration. a Cylinder test in which each mouse obtained a percentage based on the ratio of unilateral and bilateral explorations performed in three trials, of 3 min each; the percentage was expressed as mean ± SEM of eight independent tests. b Adhesive removal test that quantifies the time in which the animals remove a label previously placed by the examiner. The time of removal was quantified in seconds and was expressed as means ± SEM of six independent animals. c The tone and muscle strength of the four limbs was evaluated by exposing the mice to the inverted grid test in which the decay time was quantified for three non-consecutive opportunities of a maximum of 60 s of duration. The results are expressed as means ± SEM of the latency to fall of six independent tests. d To integrate the three previous tests, an arbitrary score was assigned to the performance of each animal, giving the highest score to the performance of the mice injected with saline (45 points). The results are expressed in arbitrary units of motor score as the mean ± SEM of the latency to fall of six independent tests. *p < 0.05 vs the corresponding saline control; ^ϕp < 0.05 vs the corresponding wild-type (WT) mouse treated with glutamate