Fig. 4
From: Experimental necrotizing enterocolitis induces neuroinflammation in the neonatal brain
Experimental NEC is associated with neuroinflammation. a RT-qPCR (top) and Western blotting and quantification (bottom) of TNFα and IL-6. Compared to breastfed control, relative gene expression and protein levels of TNFα and IL-6 increased in NEC brains. b–d Immunohistochemistry images of microglia cells (left) in NEC and breastfed control pups using the antibody Iba1 (Table 1) and their quantification (right). Compared to breastfed control, the number of microglia increased in the hippocampus (b) and in the basal ganglia/thalamus (c). Compared to breastfed control, the number of microglia increased in the cerebral cortex (d). e–g Immunohistochemistry images of astrocytes in the NEC and breastfed control pups using the antibody GFAP (left) (Table 1) and their quantification (right). Compared to breastfed control, the number of astrocytes increased in the hippocampus (e), basal ganglia/thalamus (f), and the cerebral cortex (g). Scale bar = 100 μm