Table 1 q-PCR primer sequences applied
From: TRPV1 mediates astrocyte activation and interleukin-1β release induced by hypoxic ischemia (HI)
Primer | Forward primer 5′-3′ | Reverse primer 5′-3 |
|---|---|---|
NLRP3 | ACGAGTCCTGGTGACTTTGTAT | TAGGTCCACACAGAAAGTTCTCTTA |
Caspase1 | ATGCCGTGGAGAGAAACAAGG | CCCCTGACAGGATGTCTCCA |
ASC | ACGAGTCCTGGTGACTTTGTAT | TAGGTCCACACAGAAAGTTCTCTTA |
IL-1β | TAAAGACCTCTATGCCAACACAGT | CTGACTTGGCAGAGGACAAAG |
IL-6 | TAGTCCTTCCTACCCCAATTTCC | TTGGTCCTTAGCCACTACTTC |
TNF | CCAACAAGGAGGAGAAGTTCC | CTCTGCTTGGTGGTTTGCTAC |
IL-10 | TACTGCTAACCGACTCCTTAATGC | AGAAATCGATGACAGCGCCTC |
β-actin | CACGATGGAGGGGCCGGACTCATC | TAAAGACCTCTATGCCAACACAGT |