Table 2 Antibodies for Western blot
From: TRPV1 mediates astrocyte activation and interleukin-1β release induced by hypoxic ischemia (HI)
Antibody | Host | Company | Cat. no. | Dilution | Duration |
|---|---|---|---|---|---|
GFAP | Mouse | Cell signaling | #3670 | 1:1000 | Overnight 4 °C |
JAK2 | Rabbit | Cell signaling | #3230 | 1:1000 | Overnight 4 °C |
p-JAK2 | Rabbit | Abcam | ab32101 | 1:2000 | Overnight 4 °C |
STAT3 | Rabbit | Hangzhou HuaAn Biotech | ET1607-38 | 1:1000 | Overnight 4 °C |
p-STAT3 | Rabbit | Hangzhou HuaAn Biotech | ET1603-40 | 1:1000 | Overnight 4 °C |
β-actin | Mouse | Protein tech | HRP-60008 | 1:10000 | Overnight 4 °C |
Anti-mouse IgG-HRP | Goat | PMK Biotech | PMK-014-091 | 1:100000 | 2 h RT |
Anti-rabbit IgG-HRP | Goat | PMK Biotech | PMK-014-090 | 1:100000 | 2 h RT |