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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Hydrogen-rich saline promotes microglia M2 polarization and complement-mediated synapse loss to restore behavioral deficits following hypoxia-ischemic in neonatal mice via AMPK activation

Fig. 3

HS significantly alters expression profiles of inflammation mediators. a Representative RT-PCR photographs showing mRNA levels of IL-1β, CD86, TGFβ and YM-1 within ipsilateral cortex at 3 days following HI insult. Quantitative results of relative mRNA levels within each group. Values were normalized to β-actin. N = 4/group. b Representative photographs of double immunofluorescent staining of CD16 (green), CD206 (green), and Iba-1 (red) within the ipsilateral cortex at 3 days following HI insult. Scale bar = 20 μm. Six randomly captured images (× 20) were obtained for each section per animal. N = 4/group. c BV-2 cells treated with 1 μM HS with/without 500 ng/mL LPS or 1 μM Compound C for 2 h. The mRNA levels of IL-1β, CD86, TGFβ and YM-1 were determined with use of RT-PCR. N = 4/group. Values represent the mean ± SD, * p < 0.05, **p < 0.01, ***p < 0.001 according to ANOVA

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Journal of Neuroinflammation

ISSN: 1742-2094