Fig. 4

Depletion of microglia lowers IL1β mRNA and protein. Application of LPS to OHSCs results in a significant increase in IL1β protein in the culture medium (overall effect of LPS *p = 0.019) which is lowered in cultures pre-treated with clodronate (**p = 0.0025) (n = 5–6 per treatment group) (a). IL1β mRNA transcript is significantly upregulated in clodronate-naïve OHSCs after LPS treatment (*p = 0.02), but pre-treatment with clodronate lowers transcript levels to almost undetectable levels (****p < 0.0001) (n = 4–6 per treatment group) (b). Analysis was conducted using two-way ANOVA. Error bars = mean ± SEM