Fig. 5

Anti-inflammatory effect of APN on AβO-treated BV2 cells protected HT-22 neuronal cells from cytotoxicity. a HT-22 cells were incubated for 24 h with conditioned medium derived from cultures of BV2 cells exposed by AβO with or without pre-treatment with APN. Medium from cultures of untreated microglia served as a control. b HT-22 cells were co-cultured with AβO-exposed BV2 cells with or without pre-treatment with APN for 24 h in a transwell system. HT-22 cells co-cultured with untreated BV2 cells served as a control. c HT-22 cells were incubated for 24 h with conditioned medium derived from cultures of AβO-exposed BV2 cells with pre-treatment with APN and Compound C. d HT-22 cells were co-cultured with AβO-exposed BV2 cells with pre-treatment with APN and Compound C in a transwell system. Cell viability was evaluated by the MTT assay. Data were presented as the mean ± SEM for at least three independent experiments, and each performed in triplicates (n = 3). One-way ANOVA with Tukey’s multiple comparison test revealed a difference between groups. *p < 0.05, **p < 0.01, ***p < 0.001