Fig. 5

HDAC3 inhibition decreases Nrf2 expression and Keap1-Nrf2 interaction in db/db mice and in cultured HBMEC monolayer. a, b Representative Western blot gel images and quantification of Nrf2 protein expression in nuclear extract of the db/db and db/+ mouse brains. Histone H3 served as an equal loading control. Data are expressed as mean ± SEM.*P < 0.05 vs. db/+, ^#P < 0.05 vs. db/db, n = 4 mice per group. c, d Representative Western blot gel image and quantification of Nrf2 protein expression in nuclear extract of HBMEC cultures. Histone H3 served as an equal loading control. Data are expressed as mean ± SEM. *P < 0.05 vs. norm, ^#P < 0.05 vs. HG-IL1β, n = 4 cultures per group. e, f Representative Western blot gel images and quantification for Keap1 protein expression in the mouse brain. GAPDH served as an equal loading control. Data are expressed as mean ± SEM. *P < 0.05 vs. db/+, ^#P < 0.05 vs. db/db, n = 4 mice per group. g, h Representative Western blot images and quantification for Nrf2 level (binding with keap-1) after Co-IP with Keap1 antibody using the brain lysates. Data are expressed as mean ± SEM. *P < 0.05 vs. db/+, ^#P < 0.05 vs. db/db, n = 4 mice per group. i, j Representative Western blot gel images and quantification for Keap1 protein expression in cultured HBMEC. GAPDH served as an equal loading control. k, l Representative Western blot gel images and quantification for Nrf2 level (binding with keap-1) after Co-IP with Keap1 antibody in cultured HBMEC. Data are expressed as mean ± SEM. *P < 0.05 vs. norm, ^#P < 0.05 vs. HG-IL1β, n = 4 cultures per group