Fig. 3

CCR6 knock down prevents retinal pathology and inflammation following rTBI. a High-magnification images of representative areas of the retina showing H&E staining, RBPMS-positive cells, Iba1-positive microglia, and GFAP-positive Müller cells in the retina of wild type or CCR6−/− mice. Ganglionic cells in the RGC layer is indicated by black arrows, and pyknotic cells are indicated by red arrows. Scale bar 100 μm. b–e Histograms showing cell count or intensity of immunoreactivity of ganglionic cell loss (b), RBPMS-positive ganglionic cells from the retinal whole mount preparation (c), Iba1-positive cells (d), and GFAP immunoreactivity (e). GCL, ganglion cell layer; RBPMS, RNA-binding protein with multiple splicing; GFAP, glial fibrillary acidic protein; Iba1, ionized calcium-binding adapter molecule 1. Values are shown as mean ± SEM. *p < 0.01, **p < 0.001, ***p < 0.0001