Fig. 7

IL1β decreases cell death following NMDA-induced damage in PLX5622-treated retinas. Retinas were obtained from mice that received a diet containing PLX5622 for 2 weeks prior to a single intraocular injection of saline or IL1β at day 14 followed by a single injection of NMDA at day 15, and tissues harvested at day 16 (a–e). Sections of the retina were labeled using TUNEL (a) or antibodies to Iba1 (green; c, e). The box-plots in b and d illustrate the mean, upper extreme, lower extreme, upper quartile, and lower quartile (n ≥ 6 animals). Images were obtained from central regions of the retina (a, c) and within the optic nerve (e).Significance of difference (*p < 0.05) between treatment groups was determined using a t test. The calibration bars panels a, c, and e represent 50 μm. Abbreviations: ONL, outer nuclear layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer