Fig. 7

Enhanced acetylated histone 3 (AcH3) and histone 4 (AcH4) were responsible for LXRβ induction by inhibiting HDAC5 activity. a, b Western blot analysis revealed that upregulated expression of HDAC5 but not HDAC2 was accompanied by LXRβ reduction in ACC after CFA insult. n = 4, ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 vs. Sham group. d, e SAHA incubation led to the enhanced expression of AcH3 and AcH4 in cultured neurons. c SAHA (5 μM) incubation induced Lxrβ mRNA expression in cultured neurons, n = 5. qRT-PCR data were normalized to Gapdh expression and were expressed as induction fold relative to DMSO-treated control (dotted line), n = 10. Error bars represent SEM. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 vs. DMSO-treated control