Fig. 8

Epigenetic regulation of Lxrβ by class I/IIb HDAC inhibitor SAHA in the cortical cultured neurons. Signal-to-noise determination of acetylated histone binding sites on Lxrβ promoter. a Sequencing of ChIP-qPCR material obtained from cultured neurons showed acetylated histones (AcH3 or AcH4) bound to Lxrβ promoter I, II, and IV. Lxrβ promoter enrichment (shown as % of input) displayed alongside IgG control ChIPs for b AcH3 and c AcH4. n = 6, ^***p < 0.001 vs IgG negative control. The boxes indicated the forward or reverse promoter sites of ChIP. SAHA treatment increased the binding of AcH3 but not AcH4 on Lxrβ promoter I (d), II (e), and IV (f). Results were normalized with input levels and were expressed as enrichment relative to DMSO-treated control (dotted lines). Error bars represent SEM. n = 5, ^*p < 0.05, ^***p < 0.001 vs. DMSO-treated control