| |
Brainstem
|
Cerebellum
|
|---|
|
PID
|
PID
|
|---|
|
7
|
14
|
21
|
7
|
14
|
21
|
|---|
|
TNF-α/GADPH
|
Veh
|
1.0 ± 0.1
|
1.0 ± 0.3
|
1.0 ± 0.2
|
1.0 ± 0.2
|
1.0 ± 0.1
|
1.0 ± 0.2
|
|
MOG/Veh
|
2.5 ± 0.3
|
106.4 ± 33.3°°°
|
21.6 ± 6.5
|
1.6 ± 0.1
|
71.7 ± 19.7°°°
|
32.2 ± 14.1
|
|
MOG/PEALut
|
2.4 ± 0.4
|
52.6 ± 21.9*
|
15.9 ± 4.5
|
2.0 ± 0.2
|
31.9 ± 11.4**
|
16.5 ± 4.6
|
|
IL1-β/GADPH
|
Veh
|
1.0 ± 0.1
|
1.0 ± 0.2
|
1.0 ± 0.2
|
1.0 ± 0.1
|
1.0 ± 0.5
|
1.0 ± 0.5
|
|
MOG/Veh
|
2.4 ± 0.6
|
330.2 ± 77.8°°°
|
31.5 ± 6.9
|
2.1 ± 0.3
|
123.0 ± 78.6°
|
21.8 ± 8.8
|
|
MOG/PEALut
|
4.7 ± 2.0
|
174.7 ± 65.8**
|
23.7 ± 6.5
|
4.0 ± 0.8
|
18.8 ± 12.2*
|
14.8 ± 6.8
|
|
INFγ/GADPH
|
Veh
|
1.0 ± 0.2
|
1.0 ± 0.5
|
1.0 ± 0.4
|
1.0 ± 0.2
|
1.0 ± 0.4
|
1.0 ± 0.2
|
|
MOG/Veh
|
1.0 ± 0.3
|
115.2 ± 0.5°°°
|
19.2 ± 4.7
|
1.7 ± 0.5
|
37.8 ± 15.7°°
|
27.8 ± 8.1
|
|
MOG/PEALut
|
1.4 ± 0.3
|
35.7 ± 17.2**
|
19.3 ± 7.7
|
1.0 ± 0.1
|
6.9 ± 1.8*
|
22.5 ± 10.4
|
|
NLRP3/GADPH
|
Veh
|
1.0 ± 0.1
|
1.0 ± 0.1
|
1.0 ± 0.2
|
1.0 ± 0.1
|
1.0 ± 0.1
|
1.0 ± 0.1
|
|
MOG/Veh
|
0.7 ± 0.0
|
11.2 ± 1.4°°°
|
3.3 ± 0.6
|
0.7 ± 0.1
|
7.2 ± 2.0°°°
|
4.4 ± 0.8°
|
|
MOG/PEALut
|
1.0 ± 0.2
|
6.9 ± 2.2**
|
2.2 ± 0.6
|
0.9 ± 0.1
|
2.9 ± 0.6**
|
2.9 ± 0.6
|
- Mice were treated with Veh, MOG/Veh, or MOG/PEALut for 7, 14, and 21 days. Veh or PEALut treatments started at 11 PID. TNF-α, IL-1β, INFγ, and NLRP3 mRNAs values are reported as mean ± SEM. Data are shown as relative expression of Veh-treated values normalized to 1. n = 6 mice for each group. °p < 0.05, °°p < 0.01, and °°°p < 0.001 compared with the Veh-treated group. *p < 0.05 and **p < 0.01 compared with the MOG/Veh
