Fig. 5

hIgG (2 g/kg) modulates inflammation in the spinal cord after SCI. a, b Representative images of the membrane for various cytokines in the injured spinal cord after treatment with either control buffer or hIgG (2 g/kg). Changes in cytokines were assessed with the R&D ELISA Proteome Profiler array, which compares the relative levels of 29 cytokines. c Changes to cytokines expressed in the injured spinal cord in response to treatment The protein levels were normalized to time-matched sham (laminectomy only) controls. Levels of IL-10, CX₃CL1, IL-1β, and TNF-α were significantly higher after hIgG (2 g/kg) treatment, but levels of CINC-2α/β, CINC-3, and IL-13 were significantly lower. d Relative differences of the 29 cytokines analyzed by the R&D ELISA Proteome Profiler array are shown with a heat map generated using the BROAD Institute’s R implementation of Morpheus. Data are expressed as mean Log2 (fold change) and as indicated by scale bar, blue indicates minimum level (reduction) and red indicates maximum level (increase). Multiple t tests, Holm-Sidak correction was performed (^δp < 0.05). Data are presented as mean ± SEM values