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Table 2 List of primary antibodies used for immunohistochemistry, concentration, incubation parameters, and purpose

From: The effects of human immunoglobulin G on enhancing tissue protection and neurobehavioral recovery after traumatic cervical spinal cord injury are mediated through the neurovascular unit

Antibody Concentration Incubation time and conditions Application
Rabbit anti-rat polymorphonuclear cells (PMN) conjugated with FITC (Cedarlane; CLFAD51140) 1:100 Overnight; 4 °C Neutrophil infiltration
Rabbit anti-rat Iba-1 (Wako Laboratory Chemicals; 019-19741) 1:300 Overnight; 4 °C Resident microglia
Lycopersicon esculentum (tomato) (FITC conjugated; Sigma; L0401) 1:500 Overnight; 4 °C Blood vessel
Rabbit anti-GFAP (Millipore; AB5804) 1:500 Overnight; 4 °C Astrocytes
Mouse anti-NG2 chondroitin sulfate proteoglycan antibody (Millipore; AB5384) 1:250 Overnight; 4 °C Pericytes
Rabbit PDGF receptor beta antibody [Y92] (Abcam; ab32570) 1:250 Overnight; 4 °C Pericytes
Rabbit anti-rat VCAM-1; Abcam; AB134047 1:250 Overnight; 4 °C Vascular adhesion molecule
Goat anti-human IgG (H+L) (cross-adsorbed secondary antibody AlexFluor 568) (Fisher-Invitrogen) 1:150 2 h; room temperature Human IgG