Table 2 List of primary antibodies used for immunohistochemistry, concentration, incubation parameters, and purpose

Rabbit anti-rat polymorphonuclear cells (PMN) conjugated with FITC (Cedarlane; CLFAD51140)

1:100

Overnight; 4 °C

Neutrophil infiltration

Rabbit anti-rat Iba-1 (Wako Laboratory Chemicals; 019-19741)

1:300

Overnight; 4 °C

Resident microglia

Lycopersicon esculentum (tomato) (FITC conjugated; Sigma; L0401)

1:500

Overnight; 4 °C

Blood vessel

Rabbit anti-GFAP (Millipore; AB5804)

1:500

Overnight; 4 °C

Astrocytes

Mouse anti-NG2 chondroitin sulfate proteoglycan antibody (Millipore; AB5384)

1:250

Overnight; 4 °C

Pericytes

Rabbit PDGF receptor beta antibody [Y92] (Abcam; ab32570)

1:250

Overnight; 4 °C

Pericytes

Rabbit anti-rat VCAM-1; Abcam; AB134047

1:250

Overnight; 4 °C

Vascular adhesion molecule

Goat anti-human IgG (H+L) (cross-adsorbed secondary antibody AlexFluor 568) (Fisher-Invitrogen)

1:150

2 h; room temperature

Human IgG