Fig. 4

Long-term exposure to LPS in organotypic spinal slices is not altering GABAergic currents. a Representative traces of spontaneous PSCs recorded from control (in black) and LPS 24H (in orange) ventral interneurons. The box plot shows a significant increase in PSC frequency upon LPS 24H. **P = 0.003, Mann-Whitney test. b Pharmacologically isolated IPSCs are recorded from control (in black) and LPS 24H (in orange) ventral interneuron (same cells as in a). The box plot summarizes the IPSC frequency from pooled experiments and exhibits a significantly increased frequency brought about by LPS 24H. **P = 0.007, Mann-Whitney test. c The box plot summarizes the values of τ for control and LPS; in the inset, averaged scaled and superimposed traces of the two conditions are shown. Note that no changes were detected in IPSC decay time constant. d Left, representative image of the LPS 24H-treated organotypic culture labeled with GFAP (green) and Iba1 (red). Right, high magnification Iba1 micrographs are shown (middle), and plots summarize the morphology changes of microglia upon LPS 24H. Note the significant increase in dendrites length (top) and in the transformation index (bottom); ***P < 0.001, Mann-Whitney test