Fig. 2

Cytokine profile of PBLs of AD patients before PRP treatment (t = 0). Changes in spontaneous and VSV-induced cytokine production by PBLs depending on the PBLs resistance/level of innate immunity. Freshly isolated PBLs (1 × 10⁶ /ml) from blood samples of AD patients before PRP treatment were divided in two groups. One was cultured uninfected and second was infected with VSV (10² TCID₅₀/ml). After 40 min of VSV adsorption at RT, PBLs were washed with culture medium. All cells were next incubated at 37 °C for 24 h. Samples of medium above cultured uninfected (spontaneous cytokine production) and VSV-infected PBLs were collected for cytokine measurement with ELISA. Results have been analyzed with the Student t test for two groups on log-transformed data