Fig. 6
Top genes enriched in CD11b+CD45hi cells reflected peripheral myeloid cell profiles in WD-fed mice. a, b Normalized gene expression plot (reproduced from ImmGen datasets, see the “Methods” section) showing relative gene expression values for 34 CD11b+CD45lo cell-enriched DE genes (a) or 73 CD11b+CD45hi cell-enriched DE genes (b) within macrophage (green), monocytes (pink), and neutrophils (red). Normalized expression means log2(gene expression value/average expression value of all genes). ImmGen immune cell type abbreviations: MF = macrophage, Mo = monocytes, GN = neutrophils, PC = peritoneal cavity, Bl = blood, Sp = spleen, BM = bone marrow, Alu_Lu = alveolar cell in the lung, Fem = female, CNS = central nervous system. (c) Fold change (FC) of top 10 CD11b+CD45hi cell-enriched genes (FC compared to CD11b+CD45lo cells) in WD-fed mice, colored by the significance level using -log10(FDR). d Numbers of genes involved in GO term of Biological Process and cellular component analysis based on 73 CD11b+CD45hi cell-related genes in WD-fed mice, colored by significance level using -log10(BJ). e Gene interaction network generated in IPA containing 37 of the 73 CD11b+CD45hi cell-enriched genes in WD-fed mice. f Normalized expression levels of Itgal (blood-derived immune cell marker), Cxcl2, Spp1, Stfa2l1 (specific neutrophil marker), and Aif1 (brain myeloid cell marker) across macrophage, monocytes, and granulocytes reproduced from ImmGen datasets (same datasets as a and b)