Fig. 3

CEBPD correlates to PDGFA expression in U373MG and T98G spheroid cells. a Microarray analysis performed using total RNA harvested from U373MG cells with transiently knockdown of CEBPD by siRNA. In Gene Ontology databases, the differentially expressed genes of U373MG cells transfected with siRNA are listed. b–e CEBPD deficiency impairs PDGFA expression with IL-1β treatment in U373MG or T98G spheroid cells. Western blot analyses performed using protein lysates harvested from U373MG or T98G spheroid cells stably expressing shLacZ or shC7 treated with or without 5 ng/mL IL-1β on day 3 and 6. Expression of α-tubulin served as the internal control. Cells transduced with shLacZ served as the sham control. (Total data were obtained from three independent experiments, and the data shown here were from one representative assay. All data are expressed as mean ± SEM. Differences among groups were determined with one-way ANOVA followed by Tukey’s multiple comparison test. **p < 0.01, ***p < 0.001) shLacZ, shβ-galactosidase; shC7, shCEBPD