Clinical and immunological control of experimental autoimmune encephalomyelitis by tolerogenic dendritic cells loaded with MOG-encoding mRNA

Table 3 Cytokine secretion profile of splenocytes following restimulation with MOG_35–55

The cytokine secretion profile upon restimulation with MOG_35–55, with particular focus on Th1, Th2, and Th17 cytokines, was analyzed during the in vivo model. For this, mice from each treatment group were sacrificed at different time points, allowing to analyze the kinetics of cytokine secretion along the course of EAE. The fold increase in cytokine secretion following MOG_35–55 restimulation was calculated by comparison to the negative control (unstimulated splenocytes). Results are expressed as the mean fold increase from three replicates ± standard deviation. In case the cytokine concentration was below the detection limit in the negative control, no fold increase could be calculated. Mean fold increases of > 2 log are indicated in bold. EP electroporation, p.i. post induction, B.D.L. protein concentration below detection limit in the MOG_35–55-restimulated condition

ISSN: 1742-2094