Fig. 5

Validation of gene expression of microglial- and macrophage-specific genes. a Level of mRNA expression of microglial-specific genes. b Level of mRNA expression of macrophage-specific genes. Cells obtained from the brains of TMEV-infected (n = 10) and PBS-injected (n = 20) mice, and from the spleens of TMEV-infected (n = 5) and PBS-injected (n = 5) mice were cell-sorted as follows: microglia (CD45^lo/int CD11b⁺), macrophages (CD45^hi CD11b⁺), brain lymphocytes (CD45⁺ CD11b^lo/int), and splenic T cells (CD45⁺ CD3⁺). RNA was extracted and quantified by qPCR. Levels were normalized to β-actin. Data are presented as means ± standard deviation. Shown is one of two independent experiments