Fig. 2
From: The retinal environment induces microglia-like properties in recruited myeloid cells
Repopulation of GFPhi and GFPlo microglia in CNS tissues of CD11cDTR/GFP:CX3CR1YFP-creER:ROSADTA mice after tamoxifen-induced DTA ablation. a Representative flow cytometry analysis of retinas confirming depletion of CX3CR1+ cells (2.5 mg tamoxifen on three alternating days) at 3 and 7 days after the last tamoxifen injection. Viable cells were gated on CD45medCD11b+Ly6G− myeloid cells. b Number of myeloid cells counted by flow cytometry in retina, brain, and optic nerve from control mice (N, no tamoxifen) and tamoxifen-depleted mice at days 1, 4, and 8 post-tamoxifen. Cell number is based on equivalent tissue volumes and given as mean ± SD. Sample number at each time point ranged from 6 to 16 for retina, 3 to 9 for the brain, and 3 to 8 for optic nerve