Fig. 5
From: The retinal environment induces microglia-like properties in recruited myeloid cells
Properties of YFPhi cells during recovery from tamoxifen-induced DTA depletion. CD11cDTR/GFP:CX3CR1YFP-creER:ROSADTA mice were given 2.5 mg tamoxifen on three alternating days and harvested at the indicated time after the last tamoxifen injection. a A substantial contribution to the repopulation of the depleted retina was made by GFPhiCD115lo cells. Flow cytometry plots of the retinas were concatenated to assist visualization of the differences in CD115 expression on GFPhi versus GFPlo cells. b At all times, a greater proportion of the CD115hi cells have the GFPlo microglia phenotype (data shown as mean ± SD, P < 0.01 at each time point). c Following ONC, CD115 remained concentrated in the CD45medSSCloGFPlo population of myeloid cells. d RT-qPCR analysis for CFS1 and IL-34. mRNA was prepared from indicated retinas (tam = tamoxifen, ONC ipsi = injured retina, ONC contra = uninjured retina). The ONC was done 5 days post-tamoxifen with retinas harvested 10 days post-ONC. *P < 0.05 compared to all other conditions