Fig. 3

CCR2⁺Mo-derived Mφ participates in parMφs but not vasMφs after vein occlusion. Ccl2 and Ccr2 real-time RT-PCRs in the occluded retina at indicated time points (a). The results were normalized to S26 expression. Values in histograms are the mean ± SEM of mRNA expression on the occluded area from 4 eyes per group (a; n = 4/group, Mann-Whitney t test, Ccl2 and Ccr2 expression at day 0 versus day 1 and versus day 3, *P = 0.0286). Representative images of the inner retina of Iba1 (green) and ColIV (red) double-labeled retinal flatmounts of occluded vein in WT (b) and in Ccr2^GFP/GFP (d) mice 3 days after vein occlusion. Quantification of the parenchymal Iba1⁺ Mφs per square millimeter (c; n = 4 WT and n = 6 Ccr2 ^GFP/GFP, Mann-Whitney t test, Iba1⁺ cells/mm² in control versus BRVO *P = 0.0143; BRVO in WT versus in Ccr2^GFP/GFP mice ^$P = 0.0246) and the number of perivascular Iba1⁺ Mφs per millimeter of vein (e, iba1⁺ cells/mm vein in control versus BRVO for WT, *P = 0,0143, and Ccr2^GFP/GFP mice, *P = 0.0095) of control retinas and in the occluded vein 3 days after the occlusion. Ccl2, chemokine ligand 2; Ccr2, C-C chemokine receptor type 2; BRVO, branch retinal vein occlusion; Iba1, ionized calcium-binding adapter molecule 1. Scale bar b and d = 10 μm. Inset = 5 μm