Fig. 4

Deletion of RelB in mature oligodendrocytes reduces the severity of EAE. a RelB was visualized in CC1-positive oligodendrocytes in cortical brain sections from WT (RelB^loxP/loxP) and RelB^ΔOLIGO mice by IF. Anti-CC1 and anti-RelB antibodies were used, and sections were counterstained with Hoechst. b–e EAE was induced in WT and RelB^ΔOLIGO mice. b, c Clinical scores were recorded for 23 days. n = 5–9 mice per group. b Multiple T tests (*p < 0.05). c T tests: day of onset (F = 1.11, p = 0.01); day of peak (F = 1.619, p = 0.65); peak score (F = 3.659, p = 0.02). d At the peak of disease, lumbar spinal cords were stained with hematoxylin and eosin (L2–L4) (left panels). Quantification of immune cells infiltration into peripheral regions of spinal cords. n = 6, T test: F = 9.212, p = 0.0321. e Flow cytometry was conducted to quantify the indicated cells in the brains. n = 3–4 mice per group, T tests