Fig. 6

Increased activation of p65 NF-κB in oligodendrocytes of RelB^ΔOLIGO mice. EAE was induced in WT (RelB^loxP/loxP) and RelB^ΔOLIGO mice. Tissues were collected at the peak of disease. a, b Sections of the lumbar spinal cords were stained with anti-p65, anti-pp65, and anti-CC1 antibodies, and counterstained with Hoechst. Higher magnifications are shown (right panels). c Quantification of pp65/CC1 double-positive cells (IF) in spinal cords of WT (RelB^loxP/loxP) and RelB^ΔOLIGO mice. Percentages were calculated as the ratio of pp65/CC1 double labeled cells per total CC1 cells measured using the cell counter tool in ImageJ. (n = 6–7 per group, T test: (F = 1.359, p = 0.04)