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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Depletion of regulatory T cells increases T cell brain infiltration, reactive astrogliosis, and interferon-γ gene expression in acute experimental traumatic brain injury

Fig. 5

DEREG mice show similar induction of neuronal injury and microgliosis marker but elevated protein levels of the reactive astrocyte marker GFAP. a Western blot of brain lysates (37.5 μg protein/sample) from ipsi- and contralesional brain tissue. Blots were probed with antibodies specific to αII-spectrin, Iba1, GFAP, or GAPDH. b CCI induces the generation of αII-spectrin breakdown products of 150 kDa, and 145 kDa in the ipsilesional brain tissue. The 120 kDa αII-spectrin fragment was not altered by CCI. No differences were found between DTx-treated DEREG mice compared to WT. c Protein expression of the microglia marker Iba1 appeared increased in the ipsilesional brain tissue after CCI. There were no alterations between DEREG and WT mice. d GFAP was induced by CCI in the ipsilesional brain tissue. Increased GFAP protein levels were found in DEREG mice compared to WT. Data are expressed as mean ± SEM, n = 11 per group, *p < 0.05, one-way ANOVA followed by Tukey’s multiple comparison test

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