Fig. 6From: Depletion of regulatory T cells increases T cell brain infiltration, reactive astrogliosis, and interferon-γ gene expression in acute experimental traumatic brain injuryDEREG mice develop exaggerated astrogliosis after CCI. a, b Images showing details of brain cryosections after immunostaining with anti-Iba1 (a) or anti-GFAP (b) to label microglia/macrophages or reactive astrocytes in the contralesional or ipsilesional cortex at 5 dpi, respectively. Images were processed for digital cell recognition after thresholding. c, d Histograms showing the numbers of Iba1 IR cells or GFAP IR cells per mm2. GFAP IR cells were markedly increased in ipsilesional brain parenchyma of DEREG compared to WT mice. Data are expressed as mean ± SEM, n = 11 per group, ****p < 0.0001, one-way ANOVA followed by Tukey’s multiple comparison test. Scale 100 μm (b)Back to article page