Fig. 1

Oral treatment with antibiotics exacerbates cuprizone-induced demyelination and oral treatment of SCFAs suppresses demyelination in the corpus callosum. a Black-Gold staining of brains from cuprizone and antibiotic-treated mice. Scale bar, 100 μm. b Myelinated area of the corpus callosum (n = 8 mice per group, pooled from two independent experiments). c Black-Gold staining of brains from cuprizone and SCFA-treated mice. Scale bar, 100 μm. d Myelinated area of the corpus callosum (n = 12 mice per group, pooled from four independent experiments). The box plot indicates the first and third quartiles and the middle line indicates the median. Whiskers indicate the minimum and maximum. *p < 0.05, ***p < 0.001