Fig. 2

Microglia are not affected by oral treatment with SCFAs. a Iba-1 staining of the corpus callosum. Scale bar, 100 μm. The lower row indicates a magnified figure of microglia. Scale bar, 20 μm. b Iba-1 staining of the corpus cerebral cortex. Scale bar, 100 μm. The lower row indicates a magnified figure of microglia. Scale bar, 20 μm. c The number of Iba-1⁺ cells in the corpus callosum (n = 6 mice per group, pooled from two independent experiments). d The number of Iba-1⁺ cells in the cerebral cortex (n = 6 mice per group, data are representative of two independent experiments). e Percentage of CD68⁺ activated microglia in the corpus callosum (n = 6 mice per group, pooled from two independent experiments). f Percentage of CD68⁺ activated microglia in the cerebral cortex (n = 6 mice per group, pooled from two independent experiments). The box plot indicates the first and third quartiles and the middle line indicates the median. Whiskers indicate the minimum and maximum. *p < 0.05, **p < 0.01