Fig. 6

Butyrate treatment enhances the differentiation of oligodendrocyte. a MBP and NF200 immunocytochemical staining of organotypic cerebellar slice cultures. Slice cultures were treated with LPC for 24 h and then cultured with normal medium. At 3, 5, and 7 days after LPC treatment, cultures were fixed and analyzed. Scale bar, 100 μm. b Myelination index (MI) of organotypic cerebellar slice cultures (n = 6 slices per group, pooled from two independent experiments). c Olig2, CC-1, and PDGFRα immunocytochemical staining of organotypic cerebellar slice cultures. Slice cultures were treated with LPC for 24 h and then cultured with normal medium. At 3, 5, and 7 days after LPC treatment, cultures were fixed and analyzed. Arrows indicate Olig2⁺CC-1⁺ mature oligodendrocytes. Arrowheads indicate Olig2⁺PDGFRα⁺ OPC. Scale bar, 100 μm. d OPC number in the organotypic cerebellar slice culture (n = 6 slices per group, pooled from two independent experiments). e Mature oligodendrocyte number in the organotypic cerebellar slice culture (n = 6 slices per group, pooled from two independent experiments). The box plot indicates the first and third quartiles and the middle line indicates the median. Whiskers indicate the minimum and maximum. *p < 0.05, **p < 0.01, ***p < 0.001