Fig. 5

Confocal microscopy for detection of CD3 and FoxP3 positive cells in the hippocampus of 3xTg AD mice, following chronic treatment (12 months) with an anti-TNFSF10 monoclonal antibody (10 μg/animal twice a month, i.p.) or vehicle (10 μg/animal twice a month, i.p.). Panel a: each picture represents a single group of treatment and illustrates either the whole hippocampus (sagittal section) or, below, magnification of CA2-CA3 areas displaying specific CD3 (green) immunofluorescence (magnifications of respective white frames) in the mice following chronic treatment (12 months) with an anti-TNFSF10 monoclonal antibody (10 μg/animal twice a month, i.p.) or vehicle (10 μg/animal twice a month, i.p.). Panel b: immunofluorescence of hippocampi for CD3 (red) and FoxP3 (green) expression and co-localization from the same animal groups as above (merge column; DAPI = nuclear staining). The respective side columns are the lower magnification samples where the areas to analyze (CA1, CA2, CA3, and CA4) were magnified (framed in a green box). WT wild-type animals (n = 5/group).; AD: 3xTg-AD animals (n = 5/group)