Skip to main content
Fig. 10 | Journal of Neuroinflammation

Fig. 10

From: Lipocalin-2 may produce damaging effect after cerebral ischemia by inducing astrocytes classical activation

Fig. 10

Expression of LCN2 in brain section of MCAO mice. The protein LCN2, its receptor 24p3R, and GFAP were co-localized by immunofluorescence on brain slices of MCAO mice. LCN2 was overexpressed in the infarcted area. Co-localization of 24P3R and LCN2 was not observed in the contralateral area. The 24p3R was widely expressed throughout the brain and was not affected by MCAO. LCN2 protein was no longer detected after LCN2 knockout, but the expression of 24P3R was not changed after LCN2 knockout, scale bar 40 μm. Antibody: mouse anti-GFAP (1:400, Cat#MAB360, Millipore), anti-mouse Alex 488 conjugated secondary antibody (1:200, Cat#ab150117, Abcam), rabbit anti-24p3R (1:200, Cat#ab124506, Abcam), anti-rabbit Alex 647 conjugated secondary antibody (1:200, Cat#ab150063, Abcam), goat anti-LCN2 (1:200, Cat#AF1857, R&D), anti-goat Alex 594 conjugated secondary antibody (1:200, Cat#ab150132, Abcam)

Back to article page

Journal of Neuroinflammation

ISSN: 1742-2094