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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Lipocalin-2 may produce damaging effect after cerebral ischemia by inducing astrocytes classical activation

Fig. 4

Anoxic activated astrocytes sorted according to iNOS expression. a The astrocytes were harvested 24 h after OGD and were grouped according to the expression of iNOS by magnetic particle labeling. b Western blot was used to detect the expression of protein iNOS and ARG1 in the two groups. The two groups had a significant difference in iNOS expression (P < 0.01) but had no difference in ARG1 expression (P = 0.52). c, d The statistical results of Image J. Antibody: rabbit anti-iNOS (1:1000, Cat#ab17945, Abcam), rabbit anti-ARG1 (1:1000, Cat#ab124917, Abcam), rabbit anti-beta-actin (1:2000, Cat#4970, Cell Signaling Technology), anti-rabbit (1:2000, Cat#14708, Cell Signaling Technology)

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Journal of Neuroinflammation

ISSN: 1742-2094