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Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: Lipocalin-2 may produce damaging effect after cerebral ischemia by inducing astrocytes classical activation

Fig. 8

Inhibition of OGD induced iNOS expression in LCN2 knockout astrocytes. The iNOS and GFAP were co-localized in primary cultured wild-type astrocytes after oxygen-glucose deprivation. iNOS did not express in LCN2 knockout astrocytes after oxygen-glucose deprivation, scale bar 200 μm. Antibody: rabbit anti-iNOS (1:200, Cat#ab17945, Abcam), anti-rabbit Alex 488 conjugated secondary antibody (1:200, Cat#ab150073, Abcam), goat anti-GFAP (1:200, Cat#ab53554, Abcam), anti-goat Alex 594 conjugated secondary antibody (1:200, Cat#ab150132, Abcam)

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