Fig. 4

Leu-Leu-OMe stimulation alone is sufficient to activate inflammasome and reduce the XIAP protein level. a Caspase-1 western blot was performed using ARPE-19 cell culture supernatants and cell lysates from four stimulation groups: non-stimulated RPE, IL-1α primed RPE, Leu-Leu-OMe (LLOMe) stimulated RPE, IL-1α primed, and LLOMe stimulated RPE. The cleaved caspase-1 (Casp-1) p20 bands were observed under LLOMe stimulation regardless of IL-1α priming. b XIAP protein levels were studied using cell lysates from the same groups, where LLOMe stimulation was able to decrease XIAP in RPE cells with or without IL-1α priming, compared to non-stimulated RPE cells or cells only primed with IL-1α (N = 3, Kruskal-Wallis, *p < 0.05)