Fig. 5

BI-1’s role in the Nrf-2 signaling pathway at 72 h post-HI. Representative picture of western blot data (a). Quantification of band intensities for NPR (b), HO-1 (c), and pNrf-2 (d). Immunofluorescence staining of Iba-1 with NPR or Nrf-2 (e, f). Green was for microglial staining, red was for NPR or Nrf-2 staining, and blue was for DAPI. Merge showed the co-localization of NPR or Nrf-2 on microglia (scale bar = 50 μm; n = 3/group). Data expressed as mean ± SD; an asterisk indicates p < 0.05 vs sham; number sign p < 0.05 vs vehicle; @ p < 0.05 vs Ad-TMBIM6 or scramble; n = 6/group using one-way ANOVA followed by Tukey’s multiple-comparison post hoc analysis