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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Dasatinib regulates LPS-induced microglial and astrocytic neuroinflammatory responses by inhibiting AKT/STAT3 signaling

Fig. 2

The effect of dasatinib on LPS-induced pro- and anti-inflammatory cytokine levels in primary microglial cells. ad Primary microglial cells were treated with vehicle (1% DMSO) or dasatinib (250 nM) for 2 h followed by LPS (200 ng/ml) or PBS for 5.5 h under low-glucose conditions. Proinflammatory cytokine levels were then measured by real-time PCR (con, n = 6; LPS, n = 6; dasatinib+LPS, n = 6). eh Primary microglia were treated with vehicle (1% DMSO) or dasatinib (250 nM) for 2 h followed by LPS (200 ng/ml) or PBS for 5.5 h under high-glucose conditions, and anti-proinflammatory cytokine levels were measured by real-time PCR (con, n = 6; LPS, n = 6; dasatinib+LPS, n = 6). ik Primary microglia were treated with vehicle (1% DMSO) or dasatinib (250 nM) for 2 h followed by LPS (200 ng/ml) or PBS for 5.5 h under low-glucose conditions, and anti-inflammatory cytokine levels were measured by real-time PCR. (con, n = 6; LPS, n = 6; dasatinib+LPS, n = 6.) One-way ANOVA with Tukey’s post hoc test was used to analyze significant differences. ***p < 0.001

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