Fig. 2

The effect of dasatinib on LPS-induced pro- and anti-inflammatory cytokine levels in primary microglial cells. a–d Primary microglial cells were treated with vehicle (1% DMSO) or dasatinib (250 nM) for 2 h followed by LPS (200 ng/ml) or PBS for 5.5 h under low-glucose conditions. Proinflammatory cytokine levels were then measured by real-time PCR (con, n = 6; LPS, n = 6; dasatinib+LPS, n = 6). e–h Primary microglia were treated with vehicle (1% DMSO) or dasatinib (250 nM) for 2 h followed by LPS (200 ng/ml) or PBS for 5.5 h under high-glucose conditions, and anti-proinflammatory cytokine levels were measured by real-time PCR (con, n = 6; LPS, n = 6; dasatinib+LPS, n = 6). i–k Primary microglia were treated with vehicle (1% DMSO) or dasatinib (250 nM) for 2 h followed by LPS (200 ng/ml) or PBS for 5.5 h under low-glucose conditions, and anti-inflammatory cytokine levels were measured by real-time PCR. (con, n = 6; LPS, n = 6; dasatinib+LPS, n = 6.) One-way ANOVA with Tukey’s post hoc test was used to analyze significant differences. ***p < 0.001