Fig. 3

Spatial distribution of fluorescent cells relative to the tumor microenvironment. a Five color intravital 2P microscopy of a Bev-treated triple transgenic mouse at D21 (upper left) and D28 (upper right, arrows show the LysM-EGFP⁺ cells present in the vessels). Maximal intensity projection over 150 μm depth. Scale bar 200 μm. Zoom in of the outlined regions of interest from D21 (lower left, scale bar 100 μm) and D28 (lower middle and right, scale bar 50 μm). The cells colored in pink refer to double-labeled cells (pink arrows) (CD11c-EYFP⁺/LysM-EGFP⁺) inside the tumor. b Graph showing the density of CD11c-EYFP⁺ (yellow) and LysM-EGFP⁺ (green) cells as a function of tumor depth for Bev-treated mice. *p = 0.03. Images at D21 of a Bev-treated mouse showing the distribution of LysM-EGFP⁺ cells (green) and CD11c-EYFP⁺ cells (yellow) as a function of tumor depth (150 μm), starting from the top of the tumor (right panel, scale bar 200 μm)